6E4B
The crystal structure of a putative alpha-ribazole-5'-P phosphatase from Escherichia coli str. K-12 substr. MG1655
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-06 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.114, 53.772, 199.133 |
| Unit cell angles | 90.00, 98.09, 90.00 |
Refinement procedure
| Resolution | 46.172 - 2.550 |
| R-factor | 0.1949 |
| Rwork | 0.193 |
| R-free | 0.22760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ij5 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.449 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.500 | 2.590 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.098 | 0.720 |
| Rmeas | 0.118 | 0.891 |
| Rpim | 0.064 | 0.518 |
| Number of reflections | 38300 | 1779 |
| <I/σ(I)> | 12.65 | 1.52 |
| Completeness [%] | 96.8 | 90.9 |
| Redundancy | 3.1 | 2.4 |
| CC(1/2) | 0.564 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2 M magnesium chloride, 0.1 M Tris base/Hydrochloric acid. 20% (w/v) PEG 8000 |
