6E3J

Human Bfl-1 in complex with the Bfl-1-specific designed peptide srt.F10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97910
Spacegroup name	P 1 21 1
Unit cell lengths	43.266, 42.907, 47.029
Unit cell angles	90.00, 114.73, 90.00

Refinement procedure

Resolution	42.716 - 1.482
R-factor	0.1459
Rwork	0.143
R-free	0.17780
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.510
High resolution limit [Å]	1.480	4.020	1.480
Rmerge	0.059	0.039	0.310
Rmeas	0.065	0.043	0.366
Rpim	0.026	0.017	0.189
Number of reflections	25487	1358	1008
<I/σ(I)>	7.2
Completeness [%]	97.5	98.9	80.3
Redundancy	5.4	6.2	2.9
CC(1/2)		0.998	0.864

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	1.8 M ammonium sulfate, 0.1 M MES pH 7.0