6E28

The CARD9 CARD domain-swapped dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	43.980, 37.430, 56.880
Unit cell angles	90.00, 101.47, 90.00

Refinement procedure

Resolution	43.102 - 1.360
R-factor	0.1805
Rwork	0.180
R-free	0.19370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lwd
RMSD bond length	0.009
RMSD bond angle	1.011
Data reduction software	XDS (Nov. 1, 2016)
Data scaling software	XSCALE (Nov. 1, 2016)
Phasing software	PHASER (2.8.0)
Refinement software	PHENIX ((1.12-2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.102	1.394
High resolution limit [Å]	1.360	1.360
Rmerge	0.039
Number of reflections	37569	1934
<I/σ(I)>	21.66
Completeness [%]	95.6	70.1
Redundancy	8.56
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	292	1.1 M Ammonium tartrate dibasic, pH 7.0