6E27
The CARD9 CARD domain-swapped dimer with a zinc ion bound to one of the two zinc binding sites
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.010, 37.460, 56.960 |
| Unit cell angles | 90.00, 101.79, 90.00 |
Refinement procedure
| Resolution | 43.082 - 1.811 |
| R-factor | 0.2117 |
| Rwork | 0.207 |
| R-free | 0.25750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.048 |
| Data reduction software | XDS (Nov 1, 2016) |
| Data scaling software | XSCALE (Nov 1, 2016) |
| Phasing software | PHASER (2.8.0) |
| Refinement software | PHENIX (1.12-2829_final) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.094 | 1.864 |
| High resolution limit [Å] | 1.811 | 1.811 |
| Number of reflections | 16495 | |
| <I/σ(I)> | 14.06 | |
| Completeness [%] | 98.1 | |
| Redundancy | 6.64 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 5% v/v Tacsimate, 0.1 M HEPES pH 7.0, 10% w/v Polyethylene glycol monomethyl ether 5,000 |
