6E09
Crystal Structure of Helicobacter pylori TlpA Chemoreceptor Ligand Binding Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 34-ID |
Synchrotron site | APS |
Beamline | 34-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-18 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.501, 72.354, 132.921 |
Unit cell angles | 90.00, 93.26, 90.00 |
Refinement procedure
Resolution | 26.703 - 2.300 |
R-factor | 0.192 |
Rwork | 0.190 |
R-free | 0.24640 |
Structure solution method | SIRAS |
RMSD bond length | 0.014 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.150 | 1.750 |
Rpim | 0.059 | 0.703 |
Number of reflections | 97102 | |
<I/σ(I)> | 13.2 | 4.3 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 23 | |
CC(1/2) | 0.998 | 0.948 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 19% PEG 3350, 0.15M AmNO3, 0.15M NaCl, 25mM HEPES pH 7.5, 2mM DTT |