6DZX
Crystal structure of the N. meningitides methionine-binding protein in its D-methionine bound conformation.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-18 |
| Detector | DECTRIS PILATUS3 R CdTe 300K |
| Wavelength(s) | 0.97964 |
| Spacegroup name | P 1 |
| Unit cell lengths | 79.663, 87.933, 91.639 |
| Unit cell angles | 114.83, 104.12, 105.39 |
Refinement procedure
| Resolution | 39.669 - 1.678 |
| R-factor | 0.1777 |
| Rwork | 0.176 |
| R-free | 0.20120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.108 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.670 | 39.670 | 1.710 |
| High resolution limit [Å] | 1.680 | 9.190 | 1.680 |
| Rmerge | 0.132 | 0.052 | 4.454 |
| Rmeas | 0.137 | 0.054 | 4.635 |
| Rpim | 0.036 | 0.014 | 1.249 |
| Total number of observations | 3103914 | 20801 | 143999 |
| Number of reflections | 219506 | 1367 | 10395 |
| <I/σ(I)> | 10.5 | 42 | 0.7 |
| Completeness [%] | 96.4 | 98.3 | 92.2 |
| Redundancy | 14.1 | 15.2 | 13.9 |
| CC(1/2) | 0.999 | 0.999 | 0.294 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | contains 2.3M (NH4)2SO4, 0.1M sodium acetate pH 5 |
