6DZR

Crystal structure of h38C2 K99R mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 65
Unit cell lengths	91.699, 91.699, 109.011
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.087 - 2.400
R-factor	0.1838
Rwork	0.182
R-free	0.22770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1axt
RMSD bond length	0.012
RMSD bond angle	1.259
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.100	2.480
High resolution limit [Å]	2.400	2.400
Number of reflections	20372
<I/σ(I)>	41
Completeness [%]	95.0
Redundancy	20

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		278	20% PEG 3350, 200 mM Ammonium Acetate, 40 mM Ammonium Sulfate