6DYX

Structure of VHH R419 isolated from a pre-immune phage display library

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-07
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	P 65 2 2
Unit cell lengths	58.281, 58.281, 155.358
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.145 - 1.500
R-factor	0.1767
Rwork	0.176
R-free	0.19030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dba
RMSD bond length	0.007
RMSD bond angle	0.875
Data reduction software	xia2
Data scaling software	SCALA (3.3.20)
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.145	36.145	1.540
High resolution limit [Å]	1.500	6.710	1.500
Rmerge		0.223	0.335
Rmeas	0.218	0.240	0.359
Rpim	0.067	0.084	0.126
Number of reflections	25716	349	1696
<I/σ(I)>	10.7	2.5	2
Completeness [%]	99.2	95.7	91.5
Redundancy	10.1	8.1	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.2 M ammonium sulfate 0.1 M MES 30% PEG MME