6DYO

C-terminal condensation domain of Ebony in complex with L-Dopamine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	44.967, 57.195, 50.427
Unit cell angles	90.00, 114.00, 90.00

Refinement procedure

Resolution	46.070 - 2.840
R-factor	0.2033
Rwork	0.200
R-free	0.25850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dym
RMSD bond length	0.002
RMSD bond angle	0.448
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.070	2.990
High resolution limit [Å]	2.840	2.840
Number of reflections	5590
<I/σ(I)>	6.1
Completeness [%]	99.1
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	PEG 400, HEPES, CaCl2