6DY3

Caenorhabditis elegans N-acylethanolamine-hydrolyzing acid amidase (NAAA) ortholog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-23
Detector	RAYONIX MX-300
Wavelength(s)	0.97
Spacegroup name	P 43
Unit cell lengths	89.614, 89.614, 207.058
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.824 - 2.700
R-factor	0.1705
Rwork	0.168
R-free	0.21530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5u7z
RMSD bond length	0.004
RMSD bond angle	0.611
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	45129
<I/σ(I)>	23.4
Completeness [%]	100.0
Redundancy	15.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.2 M KNO3, 0.1M BIS-TRIS propane pH 8 and 20 % PEG 3350