6DXU
Crystal Structure of Parabacteroides merdae Beta-Glucuronidase (GUS)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97942 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.371, 163.393, 120.607 |
| Unit cell angles | 90.00, 103.35, 90.00 |
Refinement procedure
| Resolution | 29.630 - 1.900 |
| R-factor | 0.1537 |
| Rwork | 0.153 |
| R-free | 0.18420 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.908 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.630 | 29.630 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 269582 | |
| <I/σ(I)> | 15.57 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | 8% PEG Monomethyl-ether 5000 0.1M Bicine pH 8.5 5mM Mannuronic Acid Pentamer |
