6DXJ
Crystal structure of double-stranded DNA GAGGCCTC; crystals grown in Zn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.078 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 44.174, 44.174, 24.281 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.650 |
| R-factor | 0.21607 |
| Rwork | 0.214 |
| R-free | 0.25535 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.817 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.052 | 0.235 |
| Number of reflections | 3080 | 301 |
| <I/σ(I)> | 10.4 | |
| Completeness [%] | 97.1 | 99 |
| Redundancy | 10.4 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 1 mM ds DNA with 5 mM zinc acetate incubated against 55% (v/v) 2-methyl-2,4-pentanediol (MPD) |
