6DXI
Crystal structure of H-NOX protein from Nostoc sp V52W/L67W double-mutant.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 65.172, 65.172, 103.264 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.20972 |
| Rwork | 0.207 |
| R-free | 0.25943 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 6DX6 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.865 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | FFT |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.139 | 0.910 |
| Number of reflections | 8033 | 964 |
| <I/σ(I)> | 9.1 | 2.1 |
| Completeness [%] | 98.0 | 99 |
| Redundancy | 4.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 289 | sodium phosphate, potassium phosphate |
