6DX2
Crystal structure of the viral OTU domain protease from Dera Ghazi Khan virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 |
| Unit cell lengths | 68.417, 68.417, 69.769 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.021 - 1.614 |
| R-factor | 0.1809 |
| Rwork | 0.179 |
| R-free | 0.21270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6dx1 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.259 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.650 |
| High resolution limit [Å] | 1.614 | 1.614 |
| Number of reflections | 40786 | 3932 |
| <I/σ(I)> | 24.8 | 1.13 |
| Completeness [%] | 99.0 | |
| Redundancy | 4.9 | |
| CC(1/2) | 0.990 | 0.568 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M citric acid pH 3.5, 13% PEG 6000 |
