6DX0

Hermes transposase deletion dimer complex with (A/T) DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	95
Detector technology	CCD
Collection date	2016-08-19
Detector	RAYONIX MX300-HS
Wavelength(s)	1.00000
Spacegroup name	C 2 2 21
Unit cell lengths	121.600, 135.500, 102.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.438 - 2.900
R-factor	0.2292
Rwork	0.225
R-free	0.30460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4d1q
RMSD bond length	0.008
RMSD bond angle	1.001
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.980
High resolution limit [Å]	2.900	2.900
Rmerge	0.077	1.016
Number of reflections	17367	1395
<I/σ(I)>	14.16
Completeness [%]	90.5
Redundancy	7.19

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	50 mM MES, 50 mM sodium acetate, 15% PEG MME 2000