6DWZ
Hermes transposase deletion dimer complex with (C/G) DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2013-02-20 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 96.380, 121.110, 132.190 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.513 - 3.200 |
R-factor | 0.2051 |
Rwork | 0.203 |
R-free | 0.29820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4d1q |
RMSD bond length | 0.017 |
RMSD bond angle | 1.735 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.980 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.124 | 1.862 |
Number of reflections | 34628 | 2544 |
<I/σ(I)> | 18.63 | |
Completeness [%] | 99.0 | |
Redundancy | 19.27 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 50 mM MES, 50 mM sodium acetate, 15% PEG MME2000 |