6DWV
Crystal structure of the LigJ Hydratase in the Apo state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-11-16 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.170, 78.220, 135.690 |
| Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
| Resolution | 26.140 - 2.200 |
| R-factor | 0.2082 |
| Rwork | 0.204 |
| R-free | 0.27810 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.113 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.140 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 83173 | |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 96.0 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 0.1 M sodium malonate 12% (w/v) polyethyleneglycol 3350 |
