6DWC
Structure of the apo 4497 antibody Fab fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-01-30 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.360, 109.670, 156.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 89.860 - 2.270 |
R-factor | 0.1857 |
Rwork | 0.184 |
R-free | 0.21690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4kvn |
RMSD bond length | 0.010 |
RMSD bond angle | 1.170 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 156.750 | 2.390 |
High resolution limit [Å] | 2.270 | 2.270 |
Rmerge | 0.038 | 0.334 |
Number of reflections | 52278 | 7553 |
<I/σ(I)> | 30.6 | 4.9 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 6.5 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.16M Calcium Acetate, 0.08 M Sodium Cacodylate, pH 6.5, 14.4% PEG8000, and 20% glycerol |