6DVD
Crystal structure of Mycobacterium tuberculosis transcription initiation complex(ECF sigma factor L) with 6 nt spacer and bromine labelled in position "-11
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-03 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 142.135, 161.485, 239.367 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.899 - 3.899 |
R-factor | 0.2216 |
Rwork | 0.220 |
R-free | 0.24610 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.461 |
Data reduction software | iMOSFLM |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.970 |
High resolution limit [Å] | 3.900 | 3.900 |
Rmerge | 0.110 | 0.768 |
Number of reflections | 44608 | |
<I/σ(I)> | 4.6 | |
Completeness [%] | 87.4 | 68.5 |
Redundancy | 6 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 295 | 100 mM sodium citrate tribasic dihydrate, pH 6.2, 200 mM sodium acetate, and 10% PEG4000 |