6DUE
Toxoplasma gondii MyoA, a Class-XIV myosin, in the pre-powerstroke state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-07-20 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.250, 96.230, 92.050 |
| Unit cell angles | 90.00, 110.98, 90.00 |
Refinement procedure
| Resolution | 48.717 - 2.600 |
| R-factor | 0.2216 |
| Rwork | 0.220 |
| R-free | 0.26120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4byf |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.950 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.124 | 0.856 |
| Number of reflections | 24306 | 3495 |
| <I/σ(I)> | 7.3 | 1.6 |
| Completeness [%] | 97.6 | 96.7 |
| Redundancy | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 1:1 protein solution to reservoir solution (22% PEG8000, 200 mM ammonium sulfate, 100 mM MES, pH 6.5), cryoprotectant: reservoir solution + 15% glycerol |
