6DU0
Crystal structure of eukaryotic DNA primase large subunit iron-sulfur cluster domain Y395L mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-08 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.462, 50.830, 90.175 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.280 - 1.820 |
| R-factor | 0.276 |
| Rwork | 0.273 |
| R-free | 0.32410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6dtz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.156 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.7.17) |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.900 |
| High resolution limit [Å] | 1.830 | 3.940 | 1.830 |
| Rmerge | 0.084 | 0.060 | 0.380 |
| Rmeas | 0.099 | 0.071 | 0.462 |
| Rpim | 0.053 | 0.038 | 0.258 |
| Total number of observations | 97436 | ||
| Number of reflections | 29372 | 3042 | 2624 |
| <I/σ(I)> | 10.7 | ||
| Completeness [%] | 95.6 | 98.6 | 85.6 |
| Redundancy | 3.3 | 3.5 | 2.6 |
| CC(1/2) | 0.992 | 0.876 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 100 mM TRIS 8.5, 40-60% MPD |
