6DU0

Crystal structure of eukaryotic DNA primase large subunit iron-sulfur cluster domain Y395L mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-08
Detector	RAYONIX MX-300
Wavelength(s)	0.97856
Spacegroup name	P 21 21 21
Unit cell lengths	38.462, 50.830, 90.175
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.280 - 1.820
R-factor	0.276
Rwork	0.273
R-free	0.32410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dtz
RMSD bond length	0.010
RMSD bond angle	1.156
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.7.17)
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.900
High resolution limit [Å]	1.830	3.940	1.830
Rmerge	0.084	0.060	0.380
Rmeas	0.099	0.071	0.462
Rpim	0.053	0.038	0.258
Total number of observations	97436
Number of reflections	29372	3042	2624
<I/σ(I)>	10.7
Completeness [%]	95.6	98.6	85.6
Redundancy	3.3	3.5	2.6
CC(1/2)		0.992	0.876

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	100 mM TRIS 8.5, 40-60% MPD