6DTM

Crystal Structure of Helicobacter pylori TlpA Chemoreceptor Ligand Binding Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-25
Detector	APEX II CCD
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	143.220, 67.830, 29.415
Unit cell angles	90.00, 94.05, 90.00

Refinement procedure

Resolution	29.342 - 2.500
R-factor	0.2275
Rwork	0.222
R-free	0.32300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.647
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.300	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.129	0.781
Rpim	0.049	0.397
Number of reflections	16013
<I/σ(I)>	10.3	1.9
Completeness [%]	97.3	91.3
Redundancy	7.3
CC(1/2)	0.996	0.788

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	depends on crystal