6DTG
Crystal structure of Haemophilus influenzae OppA complex with YLGANGRGGGS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-14 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0782 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.088, 90.921, 108.734 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.660 - 1.900 |
| R-factor | 0.1715 |
| Rwork | 0.170 |
| R-free | 0.20890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.403 |
| Data reduction software | XDS (Jan 26, 2018) |
| Data scaling software | Aimless (0.6.2) |
| Phasing software | BALBES (1.0.0) |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.660 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.080 | 0.880 |
| Number of reflections | 39899 | |
| <I/σ(I)> | 15.6 | 2.6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 8.8 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1M sodium acetate pH 4.6, 2.5M ammonium sulfate |
