6DSV

Bst DNA polymerase I post-chemistry (n+2) structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-02-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	87.082, 93.069, 104.736
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.541 - 1.990
R-factor	0.1939
Rwork	0.193
R-free	0.22840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.844
Data reduction software	autoXDS
Data scaling software	autoXDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.000	2.061
High resolution limit [Å]	1.990	1.990
Rmerge	0.327	1.745
Number of reflections	58993	5832
<I/σ(I)>	9.75
Completeness [%]	99.9
Redundancy	7.2
CC(1/2)	0.991	0.598

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	50% ammonium sulfate, 0.1M MES, 2% MPD