6DSU

Bst DNA polymerase I pre-insertion complex structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-02-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	87.440, 93.386, 104.959
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.693 - 1.980
R-factor	0.2231
Rwork	0.222
R-free	0.27160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.602
Data reduction software	autoXDS
Data scaling software	autoXDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.000	2.051
High resolution limit [Å]	1.980	1.980
Rmerge	0.057	0.241
Number of reflections	60008	5901
<I/σ(I)>	22.78
Completeness [%]	98.3
Redundancy	7
CC(1/2)	0.999	0.977

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	2.5% MPD, 52% ammonium sulfate, 0.1M MES (pH 5.8), 0.01M magnesium chloride