6DSP
LsrB from Clostridium saccharobutylicum in complex with AI-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-23 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.18076 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 90.780, 90.780, 182.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.490 - 1.370 |
| Rwork | 0.159 |
| R-free | 0.17720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tjy |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.490 | 1.390 |
| High resolution limit [Å] | 1.370 | 1.370 |
| Rmerge | 0.056 | 0.819 |
| Rmeas | 0.065 | 0.967 |
| Rpim | 0.033 | 0.509 |
| Number of reflections | 160886 | 7881 |
| <I/σ(I)> | 14.4 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 6.9 |
| CC(1/2) | 0.999 | 0.731 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.1 M Citric acid pH 2.75 and 26% w/v PEG 3350 |
