6DS0
Structural Determinants of Activation and Biased Agonism at the 5-HT2B Receptor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-08-04 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.033 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 59.482, 120.081, 169.468 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.560 - 3.188 |
R-factor | 0.2257 |
Rwork | 0.224 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | : 4IB4 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.595 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.290 |
High resolution limit [Å] | 3.188 | 3.200 |
Rmerge | 0.159 | 1.280 |
Number of reflections | 9751 | 800 |
<I/σ(I)> | 9.8 | 1.2 |
Completeness [%] | 93.5 | 96.5 |
Redundancy | 3.8 | 3.8 |
CC(1/2) | 0.994 | 0.391 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 100 mM Tris/HCl pH 8.0, 100 mM Sodium Formate, 30% v/v PEG400 |