6DRM
OTU domain of Fam105A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 170.170, 53.870, 41.520 |
| Unit cell angles | 90.00, 101.94, 90.00 |
Refinement procedure
| Resolution | 39.906 - 2.060 |
| R-factor | 0.2075 |
| Rwork | 0.206 |
| R-free | 0.22720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ksj |
| Data reduction software | XDS (20170923) |
| Data scaling software | XSCALE (20170923) |
| Phasing software | PHASER (2.5.2) |
| Refinement software | PHENIX (1.13) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.906 | 39.906 | 2.110 |
| High resolution limit [Å] | 2.060 | 9.210 | 2.060 |
| Rmerge | 0.039 | 0.025 | 1.032 |
| Rmeas | 0.046 | 0.031 | 1.217 |
| Number of reflections | 22648 | 270 | 1584 |
| <I/σ(I)> | 17.63 | 52.26 | 1.14 |
| Completeness [%] | 98.8 | 94.4 | 96.5 |
| Redundancy | 3.709 | 3.237 | 3.521 |
| CC(1/2) | 0.999 | 0.997 | 0.638 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 6% PEG8000, 0.1M Tris-HCl pH 8.0, 150 mM NaCl |
