6DR6
X-ray crystallographic structure of a covalent trimer derived from A-beta 17_36 containing the F20Cha point mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 123 |
| Detector technology | CCD |
| Collection date | 2018-02-09 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 58.058, 58.058, 95.609 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.645 - 2.608 |
| R-factor | 0.2459 |
| Rwork | 0.242 |
| R-free | 0.27680 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.283 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.645 | 2.720 |
| High resolution limit [Å] | 2.608 | 2.620 |
| Number of reflections | 5520 | |
| <I/σ(I)> | 57.4 | |
| Completeness [%] | 100.0 | |
| Redundancy | 75.4 | |
| CC(1/2) | 1.000 | 0.993 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296.15 | 0.1 M tris pH 7.5, ).2 M magnesium chloride, 3.9 M 1,6-hexanediol |
