6DP6
Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 80 mM Mg2+ and 75 mM K+ for 40 s at 21 C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-22 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 81.832, 37.292, 62.607 |
Unit cell angles | 90.00, 96.71, 90.00 |
Refinement procedure
Resolution | 20.080 - 1.403 |
R-factor | 0.1642 |
Rwork | 0.163 |
R-free | 0.18630 |
Starting model (for MR) | 1zbl |
RMSD bond length | 0.008 |
RMSD bond angle | 1.026 |
Data reduction software | HKL-2000 |
Data scaling software | Aimless (0.5.31) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.080 | 20.080 | 1.430 |
High resolution limit [Å] | 1.400 | 7.690 | 1.400 |
Total number of observations | 33053 | ||
Number of reflections | 33053 | 230 | 1120 |
<I/σ(I)> | 5 | ||
Completeness [%] | 89.9 | 92.7 | 62.3 |
Redundancy | 1 | 1 | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2 |