6DOK

Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 25 mM K+ for 120 s at 21 C (dataset 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	81.617, 37.433, 62.249
Unit cell angles	90.00, 96.58, 90.00

Refinement procedure

Resolution	19.950 - 1.380
R-factor	0.15
Rwork	0.149
R-free	0.17040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zbl
RMSD bond length	0.008
RMSD bond angle	1.001
Data reduction software	HKL-2000
Data scaling software	Aimless (0.5.31)
Phasing software	PHENIX
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.950	19.950	1.400
High resolution limit [Å]	1.380	7.540	1.380
Total number of observations	36837
Number of reflections	36837	144	736
<I/σ(I)>	5.4
Completeness [%]	94.5	56.6	37.9
Redundancy	1	1	1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	14% PEG3350, 20% glycerol, 200 mM potassium iodide, 5 mM calcium chloride