6DOH
Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Soak in 0.5 mM EGTA and 200 mM K+ at 21 C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-BM-B |
Synchrotron site | APS |
Beamline | 23-BM-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 81.194, 37.843, 61.829 |
Unit cell angles | 90.00, 96.15, 90.00 |
Refinement procedure
Resolution | 19.814 - 1.363 |
R-factor | 0.1697 |
Rwork | 0.168 |
R-free | 0.20000 |
Starting model (for MR) | pdbid 1ZBL |
RMSD bond length | 0.008 |
RMSD bond angle | 0.993 |
Data reduction software | HKL-2000 |
Data scaling software | Aimless (0.5.31) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.814 | 19.814 | 1.390 |
High resolution limit [Å] | 1.360 | 7.470 | 1.360 |
Number of reflections | 38071 | 225 | 1234 |
<I/σ(I)> | 4.6 | ||
Completeness [%] | 94.9 | 86.3 | 62.6 |
Redundancy | 1 | 1 | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2 |