6DND
Crystal structure of wild-type (WT) human Glutamate oxaloacetate transaminase 1 (GOT1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-21 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9787 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 91.036, 96.863, 97.871 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 68.850 - 2.100 |
R-factor | 0.21135 |
Rwork | 0.208 |
R-free | 0.26927 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ii0 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.569 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.070 | 0.320 |
Number of reflections | 3512 | |
<I/σ(I)> | 30.01 | |
Completeness [%] | 99.9 | |
Redundancy | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M Sodium Acetate Trihydrate pH 4.5, 25% w/v PEG 3,350 |