6DN1

CRYSTAL STRUCTURE OF THE FMN RIBOSWITCH BOUND TO BRX1151 SPLIT RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97950
Spacegroup name	P 31 2 1
Unit cell lengths	74.440, 74.440, 140.710
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.880 - 3.030
R-factor	0.224
Rwork	0.223
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3f4e
RMSD bond length	0.010
RMSD bond angle	0.770
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	140.711	140.711	2.170
High resolution limit [Å]	2.050	6.120	2.050
Rmerge	0.095	0.035
Rmeas	0.111	0.040
Number of reflections	36651	1804	1350
<I/σ(I)>	6.31	30.47
Completeness [%]	67.0	87.9	15.4
Redundancy	6.8
CC(1/2)	0.998	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	293.15	PEG 4000