6DMV
Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Soaked for 40 s in 2 mM Mg2+ and 200 mM K+ at 21 C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-BM-B |
| Synchrotron site | APS |
| Beamline | 23-BM-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.422, 37.803, 61.986 |
| Unit cell angles | 90.00, 96.31, 90.00 |
Refinement procedure
| Resolution | 19.881 - 1.520 |
| R-factor | 0.1624 |
| Rwork | 0.161 |
| R-free | 0.18170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6dmn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.025 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.880 | 19.880 | 1.550 |
| High resolution limit [Å] | 1.520 | 8.330 | 1.520 |
| Total number of observations | 28103 | 178 | 635 |
| Number of reflections | 28103 | 178 | 635 |
| <I/σ(I)> | 5.4 | 15 | 1.1 |
| Completeness [%] | 96.6 | 88.8 | 43.2 |
| Redundancy | 1 | 1 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2 |
