6DMN
Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Soaked in 2 mM Ca2+ and 200 mM K+ at 21 C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-BM-B |
| Synchrotron site | APS |
| Beamline | 23-BM-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.465, 37.681, 62.022 |
| Unit cell angles | 90.00, 95.93, 90.00 |
Refinement procedure
| Resolution | 19.864 - 1.270 |
| R-factor | 0.1512 |
| Rwork | 0.150 |
| R-free | 0.17140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 1ZBL |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.998 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.864 | 1.320 | 1.290 |
| High resolution limit [Å] | 1.270 | 1.290 | 1.270 |
| Number of reflections | 46733 | 2303 | 2218 |
| <I/σ(I)> | 7.2 | ||
| Completeness [%] | 94.3 | 93.3 | 90.1 |
| Redundancy | 3 | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 14% PEG3350, 20% glycerol, 200 mM KI, and 25 mM CaCl2 |
