6DLX
FtsY-NG domain bound to fragment 3.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-03-21 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.451, 76.020, 107.882 |
| Unit cell angles | 90.00, 90.41, 90.00 |
Refinement procedure
| Resolution | 43.991 - 1.848 |
| R-factor | 0.2051 |
| Rwork | 0.200 |
| R-free | 0.24930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6cqp |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.553 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.991 | 1.914 |
| High resolution limit [Å] | 1.848 | 1.848 |
| Rmerge | 0.028 | |
| Rmeas | 0.040 | |
| Rpim | 0.028 | |
| Number of reflections | 47444 | |
| <I/σ(I)> | 13.8 | |
| Completeness [%] | 99.4 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 297.15 | 0.1 M Bis-Tris pH 5.8, 25% PEG 3350, 165 mM ammonium acetate |
