6DL8
Crystal Structure of an influenza A hemagglutinin antibody Fab variant CH67:1203d4 chimera
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-23 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9775 |
| Spacegroup name | P 1 |
| Unit cell lengths | 72.235, 73.695, 76.848 |
| Unit cell angles | 62.91, 88.86, 62.07 |
Refinement procedure
| Resolution | 30.930 - 3.805 |
| R-factor | 0.2352 |
| Rwork | 0.233 |
| R-free | 0.28370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hkx |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.581 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3211: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.530 | 4.010 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.113 | |
| Number of reflections | 11641 | |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 98.1 | |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 291.16 | 15% v/v IPA, 0.1M NaCit pH 5.0, 10% PEG 10000 |
