6DKN
CRYSTAL STRUCTURE OF ARABIDOPSIS THALIANA DECAPPING NUCLEASE DXO1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.080, 80.878, 125.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.520 - 1.800 |
| R-factor | 0.20291 |
| Rwork | 0.201 |
| R-free | 0.24202 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fqg |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.368 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.058 | 0.400 |
| Number of reflections | 61925 | 8773 |
| <I/σ(I)> | 20.7 | |
| Completeness [%] | 98.9 | |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M NH4F and 18% (w/v) PEG3350 |
