6DKL

Crystal Structure of a Rationally Designed Six-Fold Symmetric DNA Scaffold

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	68.408, 68.408, 143.222
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.176 - 3.034
R-factor	0.2284
Rwork	0.228
R-free	0.23390
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.955
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.100
High resolution limit [Å]	3.034	3.034
Rmerge	0.106
Rmeas	0.115
Rpim	0.044	0.200
Number of reflections	6788	252
<I/σ(I)>	42.1
Completeness [%]	91.8
Redundancy	6.8
CC(1/2)	0.980	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	50 mM Cacodylate pH 6.0, 10 mM MgCl2, 200 mM sodium citrate, 5% isopropanol