6DK9
Yeast Ddi2 Cyanamide Hydratase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-15 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.12713 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 264.456, 264.456, 119.152 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.700 - 2.600 |
| R-factor | 0.2014 |
| Rwork | 0.201 |
| R-free | 0.21680 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.812 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.700 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.105 | 0.695 |
| Number of reflections | 144816 | 7127 |
| <I/σ(I)> | 17.6 | 2.9 |
| Completeness [%] | 99.0 | 97.9 |
| Redundancy | 7.7 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 1.1-1.3 M Ammonium Sulfate 0.2 M Arginine 0.1 M N-morpholino ethane sulfonate pH 5.6 |
