6DK7
RetS histidine kinase region with cobalt
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-04 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97881, 0.9751 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 158.708, 158.708, 243.826 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.779 - 2.600 |
R-factor | 0.2123 |
Rwork | 0.211 |
R-free | 0.26060 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.838 |
Data reduction software | xia2 |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.890 | 2.693 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.745 | |
Number of reflections | 96240 | 13834 |
<I/σ(I)> | 9.3 | |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 14.3 | 12.6 |
CC(1/2) | 0.997 | 0.273 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | sodium chloride, HEPES, cobalt chloride |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | sodium chloride, HEPES, cobalt chloride |