Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.912386 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.034, 75.029, 75.199 |
| Unit cell angles | 90.00, 108.77, 90.00 |
Refinement procedure
| Resolution | 33.560 - 2.040 |
| R-factor | 0.1983 |
| Rwork | 0.197 |
| R-free | 0.25750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ls3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.345 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.080 |
| High resolution limit [Å] | 2.040 | 5.540 | 2.040 |
| Rmerge | 0.060 | 0.042 | 0.397 |
| Total number of observations | 109928 | ||
| Number of reflections | 31273 | 1567 | 1522 |
| <I/σ(I)> | 14.3 | ||
| Completeness [%] | 98.6 | 95.5 | 96.5 |
| Redundancy | 3.5 | 3.1 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 297 | 5% PEG 8000, 0.1 M Tris-HCL pH8.0 |
