6DJ0
ASLTVS segment from Human Immunoglobulin Light-Chain Variable Domain, Residues 73-78, assembled as an amyloid fibril
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 15.167, 11.580, 18.798 |
| Unit cell angles | 90.00, 97.32, 90.00 |
Refinement procedure
| Resolution | 18.640 - 1.300 |
| R-factor | 0.1861 |
| Rwork | 0.183 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IDEAL beta strand |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.085 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 1.400 |
| High resolution limit [Å] | 1.300 | 2.220 | 1.300 |
| Rmerge | 0.192 | 0.133 | 0.270 |
| Total number of observations | 3352 | ||
| Number of reflections | 1351 | 358 | 207 |
| <I/σ(I)> | 5.6 | ||
| Completeness [%] | 80.5 | 100 | 62.5 |
| Redundancy | 2.5 | 3.2 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.1 M Malic Acid, MES:Tris buffer, 25% PEG 1500 |
