6DI7

Vps1 GTPase-BSE fusion complexed with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-30
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	84.979, 84.979, 271.502
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.895 - 2.300
R-factor	0.1784
Rwork	0.177
R-free	0.19930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2x2e
RMSD bond length	0.002
RMSD bond angle	0.532
Data reduction software	MOSFLM (7.2.1)
Data scaling software	Aimless (0.5.14)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.895	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.093	0.740
Rmeas	0.098	0.776
Rpim	0.029	0.232
Number of reflections	17235	1670
<I/σ(I)>	17.2	3.9
Completeness [%]	100.0	100
Redundancy	11	11.1
CC(1/2)	0.999	0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	14-19% PEG4000, 0.07-0.1 M trisodium citrate, 20% isopropanol