6DI6

Crystal structure of eukaryotic DNA primase large subunit iron-sulfur cluster domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0782
Spacegroup name	P 21 21 21
Unit cell lengths	41.085, 50.769, 89.832
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.940 - 1.390
R-factor	0.126
Rwork	0.124
R-free	0.14600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dtz
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHASER (2.7.17)
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	1.430
High resolution limit [Å]	1.380	1.380
Rmerge	0.096	0.312
Rmeas	0.106	0.075
Rpim	0.044	0.031
Total number of observations	206412
Number of reflections	37530	4040
<I/σ(I)>	9.3
Completeness [%]	95.3	76.5
Redundancy	5.5	4.7
CC(1/2)		0.962

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	MPD, TRIS-HCl