6DI2

Crystal structure of eukaryotic DNA primase large subunit iron-sulfur cluster domain Y397L mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0787
Spacegroup name	P 21 21 21
Unit cell lengths	40.950, 50.865, 89.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.620 - 1.320
R-factor	0.121
Rwork	0.120
R-free	0.14100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dtz
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHASER (2.7.17)
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	1.370
High resolution limit [Å]	1.320	1.320
Rmerge	0.066	0.184
Rmeas	0.072	0.073
Rpim	0.027	0.027
Total number of observations	301921
Number of reflections	44491	4720
<I/σ(I)>	11.7
Completeness [%]	99.2	94.9
Redundancy	6.8	6.4
CC(1/2)		0.985

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	MPD, TRIS-HCl