6DI2
Crystal structure of eukaryotic DNA primase large subunit iron-sulfur cluster domain Y397L mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-10 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0787 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.950, 50.865, 89.620 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.620 - 1.320 |
R-factor | 0.121 |
Rwork | 0.120 |
R-free | 0.14100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6dtz |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | PHASER (2.7.17) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.370 |
High resolution limit [Å] | 1.320 | 1.320 |
Rmerge | 0.066 | 0.184 |
Rmeas | 0.072 | 0.073 |
Rpim | 0.027 | 0.027 |
Total number of observations | 301921 | |
Number of reflections | 44491 | 4720 |
<I/σ(I)> | 11.7 | |
Completeness [%] | 99.2 | 94.9 |
Redundancy | 6.8 | 6.4 |
CC(1/2) | 0.985 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | MPD, TRIS-HCl |