6DHW

Crystal structure of primase iron-sulfur domain (266-457)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-22
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0781
Spacegroup name	C 1 2 1
Unit cell lengths	110.193, 52.560, 88.774
Unit cell angles	90.00, 115.08, 90.00

Refinement procedure

Resolution	37.197 - 2.013
R-factor	0.1821
Rwork	0.181
R-free	0.21130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3l9q
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.080
High resolution limit [Å]	2.010	4.330	2.010
Rmerge	0.117	0.060	0.648
Rmeas	0.138	0.070	0.771
Rpim	0.072	0.036	0.414
Total number of observations	99449
Number of reflections	27613	3108	2609
<I/σ(I)>	8.1
Completeness [%]	90.0	98.5	86
Redundancy	3.6	3.7	3.3
CC(1/2)		0.994	0.390

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	100 mM TRIS-HCl pH 8.5, 400 mM LiSO4, 18% PEG3350