6DHV

Structure of Arabidopsis Fatty Acid Amide Hydrolase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	93.15
Detector technology	CCD
Collection date	2017-02-19
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.1808
Spacegroup name	P 1 21 1
Unit cell lengths	72.083, 79.660, 132.592
Unit cell angles	90.00, 104.44, 90.00

Refinement procedure

Resolution	33.004 - 2.099
R-factor	0.193
Rwork	0.192
R-free	0.21960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kfu
RMSD bond length	0.003
RMSD bond angle	0.625
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.061	0.034	0.633
Rmeas	0.072	0.041	0.753
Rpim	0.038	0.021	0.403
Total number of observations	282744
Number of reflections	83848	8617	7761
<I/σ(I)>	11.9
Completeness [%]	98.9	99.2	91.9
Redundancy	3.4	3.4	3
CC(1/2)		0.998	0.773

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293.15	0.1 M MES, pH 6.0, 30% PEG 200, 5% PEG 3350