6DGV
iGABASnFR Fluorescent GABA Sensor precursor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 64 |
| Unit cell lengths | 160.970, 160.970, 50.071 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 139.400 - 2.800 |
| R-factor | 0.17777 |
| Rwork | 0.175 |
| R-free | 0.22830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4EQ7 and the cpGFP component of 3OSR |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.818 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 139.404 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.362 | 0.025 |
| Rmeas | 0.370 | 2.560 |
| Rpim | 0.080 | 0.549 |
| Number of reflections | 18621 | 2692 |
| <I/σ(I)> | 14.7 | 2.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21.5 | 21.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.056 M Sodium phosphate monobasic monohydrate 1.344 M Potassium phosphate dibasic |
